Glycosylation and methylation in the biosynthesis of isoflavonoids in Pueraria lobata (2024)

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“…Afterwards, 4-coumaroyl-CoA is converted to isoliquiritigenin by chalcone synthase and chalcone reductase [33][34][35][36][37][38]. Chalcone isomerase (CHI) then catalyzes the formation of liquiritigenin from isoliquiritigenin, which is further catalyzed by 2-hydroxyisoflavanone (IFS) to produce 2,7,4 ′ -trihydroxyisoflavonone [36,37,39].…”

confidence: 99%

Liga,

Paul

2024

Flavonoids, a variety of plant secondary metabolites, are known for their diverse biological activities. Isoflavones are a subgroup of flavonoids that have gained attention for their potential health benefits. Puerarin is one of the bioactive isoflavones found in the Kudzu root and Pueraria genus, which is widely used in alternative Chinese medicine, and has been found to be effective in treating chronic conditions like cardiovascular diseases, liver diseases, gastric diseases, respiratory diseases, diabetes, Alzheimer’s disease, and cancer. Puerarin has been extensively researched and used in both scientific and clinical studies over the past few years. The purpose of this review is to provide an up-to-date exploration of puerarin biosynthesis, the most common extraction methods, analytical techniques, and biological effects, which have the potential to provide a new perspective for medical and pharmaceutical research and development.

BACKGROUNDThe conversion of biomass into high value‐added platform compounds is an important method of biomass utilization. The conversion of hemicellulose represented by xylan into furfural can not only reduce the consumption of fossil fuels, but also promotes the development and utilization of non‐edible biomass resources. In this study, a bifunctional solid‐acid catalyst prepared from agricultural and forestry waste Pueraria (P. eduli) Residues was used to convert xylan into furfural in a biphasic system.RESULTSIn this study, P. eduli Residues was used as raw material to prepare a P. eduli Residues‐based carbon solid‐acid catalyst (PR/C‐SO3H‐Fe) by one‐step sulfonation carbonization and impregnation. The catalyst catalyzes the conversion of xylan to furfural in a biphasic system (2‐methyltetrahydrofuran/water). The physicochemical properties of the catalysts were characterized by X‐ray powder diffraction, scanning electron microscopy, differential thermogravimetric analysis, Brunauer–Emmett–Teller surface area, Fourier transform infrared spectroscopy and ammonia temperature‐programmed desorption. Subsequently, the experimental conditions were studied and optimized, such as metal species, iron ion concentration, reaction time and temperature, volume ratio of organic phase to water phase and ratio of substrate to catalyst. The results showed that under conditions of 160 °C, 50 mg catalyst, 100 mg xylan and 7 mL reaction solvent, the yield of furfural could reach 78.94% after 3 h of reaction.CONCLUSIONThis study provides an effective research method for the conversion of xylan into furfural, and provides a reference for the catalytic conversion and utilization of hemicellulose in agricultural and forestry biomass. It also provides a feasible method for the resource utilization of agricultural and forestry waste. © 2024 Society of Chemical Industry.

Kudzu, scientifically known as Pueraria montana var. lobata (Willd.) Maesen & S.M.Almeida ex Sanjappa & Predeep (P. lobata), is a perennial vine belonging to the family Leguminosae. Puerarin, a unique constituent and primary active ingredient of this genus, exhibits a broad spectrum of pharmacological activities. This study started with several practical questions: Why is the root the main medicinal part? Why is it not peeled for medicinal purposes? Why is the harvest period usually from December to February? Although the puerarin biosynthesis pathway has been investigated, the stage at which the 8-C glycosylation reaction occurs remains controversial. In this study, metabolomics and transcriptomics analyses were performed on P. lobata organs and tissues, including leaves, young stems, mature stems, tuberous cortices, and cortex-excised tubers of roots. Two modules containing genes associated with puerarin biosynthesis were identified by WGCNA. The final selection of important candidate UDP-glucosyltransferases (UGTs) that may be involved in the puerarin biosynthesis pathway included two 8-C-GTs, three 7-O-GTs, and key transcription factors. On this basis, the regulatory network of puerarin biosynthesis was constructed and laid the foundation for the cultivation of high-quality medicinal kudzu with high puerarin content.